ABSTRACT
An
assessment of the physicochemical, parameters, algal diversity and bacterial
flora of Asata River Enugu, Enugu State, Nigeria was carried out for six
months. Water samples and periphyton were collected fortnightly from four
locations namely: Okpara Coal Mine, University of Nigeria Teaching Hospital
(UNTH) Old Site, Ogbete market and Ogui and analysed using standard procedures.
Algal diversity and species richness were determined using Shannnon – Wiener
index. Spread plate method on Macconkey agar was used for bacterial count.
Results showed monthly variations (P≤0.05) in air temperature, velocity, alkalinity,
aluminum, dissolved oxygen, biological oxygen demand, chemical oxygen demand,
calcium, pH, total suspended solids, iron, lead and zinc, at the various
locations. Significant (P≤0.05) positive (colour and depth, transparency, iron
and Bacillariophyta) and negative (pH and algal divisions; lead and
Bacillariophyta; mercury and Cyanophyta) correlations were also observed among
some parameters investigated. Thirty-four algal taxa belonging to four
divisions in the following decreasing order: Bacillariophyta (33 %),
Chlorophyta (32 %), Euglenophyta (21 %), and Cyanophyta (14 %) were
encountered. Some pollution tolerant algae such as Gomphonema,
Nitzschia, Navicula, Surirella, Euglena and
Oscillatoria were dominant. Based on Shannon–Wiener index, the water
at UNTH and Ogbete were classified as mesoptrophic whereas, Okpara coal mine
and Ogui were classified as eutrophic. The diversity index showed no complete
evenness in the river. The lowest and highest enteric bacterial counts (1.029 ×
104 ± 1.174 and 5.05 × 104 ± 1.472) were observed in
August and November respectively. Gram – positive and gram – negative bacteria
such as Escherichia, Salmonella, Micrococcus, Proteus and Enterobacter
were isolated. Escherichia was dominant indicating fecal pollution. pH
was within the permissible limit; iron, lead and phosphate beyond the
limit; and calcium, magnesium, biological oxygen demand, chemical oxygen
demand, dissolved oxygen below WHO standard for drinking water, indicating that
the river was eutrophic.
TABLE OF CONTENT
TITLE PAGE
ABSTRACT
TABLE OF CONTENTS
LIST OF FIGURES
LIST OF TABLES
LIST OF PLATES
CHAPTER ONE:
INTRODUCTION
1.1 Statement of the Problem
1.2 Objectives of the study
CHAPTER TWO:
LITERATURE REVIEW
2.1 Physicochemical or Abiotic factor
2.2 Biological or Biotic Factors
2.3 Economic importance of algae
CHAPTER THREE: MATERIALS AND METHODS
3.1 Study Area
3.2 Collection of Samples
3.3 Meteorological Data
3.4 Physicochemical Methods
3.4.1 Physical methods
3.4.1.1 Temperature
3.4.1.2 Depth
3.4.1.3 Transparency
3.4.1.4 Colour
3.4.1.5 Rate of flow
3.4.2 Chemical methods
3.4.2.1 pH
3.4.2.2 Dissolved oxygen
content
3.4.2.3 Biochemical Oxygen
Demand
3.4.2.4 Chemical Oxygen
Demand
3.4.2.5 Total Alkalinity
3.4.2.6 Total Iron
3.4.2.7 Ammonia
3.4.2.8 Silica
3.4.2.9 Phosphate
3.4.2.10 Nitrate
3.4.2.11 Hydrogen Sulphide
3.4.2.12 Calcium
3.4.2.13 Magnesium
3.4.2.14 Lead
3.4.2.15 Mercury
3.5 Biological Methods
3.5.1 Algal Studies
3.5.1.2 Labeling
3.5.1.3 Preservation of
Samples
3.5.1.4 Quantitative
Evaluation of Algae
3.5.1.5 Preparation and
mounting of Slides
3.5.1.6 Microscopy
3.5.1.7 Counting Method
3.5.1.8 Diversity Index
3.5.2 Microbial Analysis of
Water
3.5.2.1 Media Preparation
3.5.2.2 Microbial Count
3.5.2.3 Microbial
Characterization
3.5.2.4 Catalase Test
3.5.2.5 Citrate utilization
test
3.5.2.6 Oxidase test
3.5.2.7 Urease test
3.5.2.8 Indole test
3.6 Data Analysis
CHAPTER FOUR:
RESULTS
4.1.1 Rainfall
4.1.2 Relative Humidity
4.1.3 Solar Radiation
4.1.4 Atmospheric Temperature
4.1.5 Wind Speed
4.2 Okpara Coal Mine
4.2.1 Physicochemical Parameters of Asata River at Okpara Coal Mine
4.2.2 Algal Biodiversity of Asata River at Okpara Coal Mine
4.2.3 Monthly Variations in Algal Densities (individual/ml) for Okpara
Coal Mine
4.3 University of Nigeria Teaching Hospital Enugu (UNTH) Old Site
4.3.1 Physicochemical Parameters of Asata River at (UNTH) Old Site
4.3.2 Algal Biodiversity of Asata River at (UNTH) Old Site
4.3.3 Monthly Variations in Algal Densities at (UNTH) Old Site
4.4 Ogbete Market
4.4.1 Physicochemical Parameters of Asata River at Ogbete Market
4.4.2 Algal Biodiversity of Asata at Ogbete Market
4.4.3 Monthly Variations in Algal Density for Ogbete Market
4.5 Ogui
4.5.1 Physicochemical
Parameters of Asata River at Ogui
4.5.2 Algal Biodiversity of
Ogui
4.5.3 Monthly Variations in
Algal Density for Ogui
4.6 Algal Check List for
Asata River
4.8. Percentage Composition
of Algae Encountered
4.9 Algal Species Diversity
and Evenness Indices
4.10 Microbial Analysis
4.10.1 Bacteriology
4.10.2 Monthly Variations in
Microbial Count
4.11 Correlation of Algae and
Physicochemical Parameters
4.12 ANOVA Result for inter –
location
4.12.1 Comparism of Water
Quality Assessment at the locations sampled
4.12.1.1 Mean Values of Some
Physicochemical Parameters at Different Locations of Asata River
4.12.1.2 Mean Values of Some
Physicochemical Parameters at Different Sampling Months
4.13 Comparism of Water
Quality of Asata River with WHO Standard
CHAPTER FIVE:
DISCUSSION
REFERENCES
CHAPTER ONE
INTRODUCTION
Rivers are
important pathways for flow of energy, matter and organisms through the
landscape. They have always provided a focus of attraction for environmental
studies. They offer a number of benefits and services to man. A wide range of
human activities at the catchment areas may lead to environmental deterioration
of river quality (Kagalou et al., 2002). The increasing
environmental pollution is alarming and is on a global scale by anthropogenic
activities.
The
productivity of the river is determined by the water quality parameters, the
aquatic species composition and abundance are affected, hence there is the need
to monitor the water quality (Agboola et al., 2011). Rivers are useful
in multiple ways such as source of water for man and for domestic animals, in
fisheries, agriculture and generation of hydroelectric power. Also tropical
rivers play a crucial role in the epidemiology of several tropical diseases (Kadiri,
2007).
The river system presents
interesting feature for aquatic organisms, in relation to extended periods of
dryness, land use practices and intense human activities, all of which
influence water quality (Papastergradou and Babalonas, 1993). Knowledge of
specific relationships between particular organisms and environmental factors
enable a quick look and fairly reliable evaluation of the essential features of
a given biotope (Fytainos et al., 2001).
Pollution of fresh water
bodies such as rivers, streams, lakes and ponds is mostly experienced as a
result of industrial discharge, municipal waste disposal and surface
Water
quality is crucial in order that man can benefit from river through series of
users (Oliveira et al., 2006). Water quality deals with physical,
biological and chemical characteristics in relation to all other hydrological
properties. Any characteristics of water that affect survival, reproduction;
growth and production of aquaculture species; influence management decisions;
cause environmental impact or reduce product quality and safety can be
considered a variable quality. The use of physicochemical properties of water
to assess water quality gives a good impression of the status, productivities,
and suitability of such water body. The change in physical characteristics like
depth, temperature, transparency and chemical element of water such as
dissolved oxygen, chemical oxygen demand, nitrate, phosphate provides valuable
information of the quality of the water, the source (s) of the variation and
biodiversity (Mustapha, 2006, 2008; Sumita et al., 2010).
Nutrients play a major role in
growth and development of plants in tropical water where there is enough light
for photosynthetic activities throughout the year, resulting in light and
temperature probably not being limiting. Aquatic autotrophs have nutritional
and energetic requirement for their photosynthetic carbon function and growth.
Nutrient limitation is important in the regulation of phytoplankton abundance
(Hirose et al., 2003). Phytoplankton diversity with the seasonal
fluctuation indicates the condition of ecological niches.
Algae are simple plants that
vary considerably in size, shape and colour and are found in a range of
habitats (Christi et al., 2011). Algae are frequently found in polluted
and unpolluted waters and due to this behavior they are considered useful in
determining the......
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