For more Human Physiology Project click here


Title page
Table of Contents
List of Abbreviations

1.0       Introduction
1.1       Statement of Research Problem
1.2       Justification of Study
1.3       General Aim of the Study
1.3.1    Specific Objectives of the Study
1.4       Research Hypothesis

2.0       Literature Review
2.1       Oxidative Stress and the Nervous System
2.2       Vitamin C in the Nervous System
2.3       Vitamin E and Memory
2.4       Sex hormones and Memory
2.4.1    Neurobiology of Oestrogen
2.4.2    Oestrogen and Cognition
2.4.3    Oestrogen and Learning and Memory
2.4.4    Testosterone and Cognition
2.4.5    Gonadal Hormones and the Cholinergic system
2.5       Cognition
2.6       Memory
2.6.1    Classification of Memory
2.7       Learning
2.8       Sex Differences in Cognitive functions

3.0       Materials and Methods
3.1       Site of experiment
3.2.1    Experimental Animals
3.2.2    Housing and grouping
3.3       Drugs and soya oil preparation
3.3.1    Drugs and preparation
3.4       Neurobehavioural Assessments
3.4.1    Elevated Plus Maze for Memory
3.4.2    Object Recognition Test
3.4.3    Object Location Test
3.5       Biochemical Tests
3.5.1    Effect of Drugs on Brain Lipid Peroxidation
3.5.2    Evaluation of Brain Catalase Activity
3.5.3    Evaluation of Brain Glutathione Peroxidase Activity
3.5.4    Evaluation of Brain Superoxide Dismutase Activity
3.6       Determination of Gonadal Hormone Levels
3.6.1    Determination of testosterone
3.6.2    Determination of Estrogen level
3.7       Statistical Analysis

4.0       RESULTS
4.1       Neurobehavioural Assessments
4.2       Elevated Plus Maze for Memory
4.3       Object Recognition Test
4.4       Object Location Test
4.5       Lipid Peroxidation Assessment
4.6       Gonadal Hormone Assay
4.7 Relationship between Serum Gonadal Hormones and Memory indices in mice

5.1       Discussion

6.1       Summary
6.2       Conclusion
6.3       Recommendations


Vitamins C and E are naturally present in some foods and are available as dietary supplements. While vitamin C is also known as L-ascorbic acid, vitamin E is a generic name for tocopherols and tocotrienols each with α, β, γ and δ subunits. Neurobehavioural models have been used to study behaviour in animals with models specific for each animal species and behaviour. The aim of the study was to investigate the effect of vitamins C and E on memory and serum biochemical changes in adult mice. Twenty male and twenty female mice weighing 16-35 g were divided into 5 groups of eight mice each. The first group served as the control and received distilled water (1 ml/kg); mice in the second group served as the positive control and received soya oil (1 ml/kg), animals in group three received vitamin C (100 mg/kg), group four received vitamin E (100 mg/kg) and the last group received both vitamins C and E (100 mg/kg). The drugs were given once daily orally for a period of 21 days. Learning and memory were assessed using the elevated plus maze (EPM), object recognition and location models for memory (ORT and OLT) at the end of the experimental period. Memory index was calculated. The mice were sacrificed on day 22 and serum estrogen and testosterone levels and catalase, superoxide dismutase and glutathione peroxidase activities were also evaluated. Lipid peroxidation was determined by measuring the malondialdehyde concentration in the brain sample. The relationship between the gonadal hormone levels and the performance of animals in each of the neurobehavioural models for memory was positive. There was no significant difference in the malondialdehyde concentration (P < 0.05) of all groups in the males but in females between the control and vitamin C-treated group. The activities of superoxide dismutase showed no significant difference in the males but showed significance (P < 0.05) between control and treatment groups in the females, catalase showed no significant difference in the females but showed significant difference (P < 0.05) between control and all groups and glutathione peroxidase showed no significant difference between all groups (P < 0.05) of both males and females. The memory index for the EPM also showed significant difference (P < 0.05) between the vitamin E-treated group and the soya oil and vitamin C-treated groups on the first day and between vitamin E and C-treated group on the second day in the females. In the males the significant difference was observed between control and treatment groups on both days. In the ORT model, discriminatory and recognitive index showed a significant (P < 0.05) difference in the vitamin E and E+C treated groups. In the OLT model discriminatory and recognitive indices showed a significant difference (P < 0.05) in the vitamin E and vitamins C+E treated groups. In conclusion, administration of vitamin C and E improved memory indices and there was a positive relationship between endogenous gonadal hormones and recognition.



Vitamin C, also known as L-ascorbic acid, is a water-soluble vitamin that is naturally present in some foods, added to others, and available as a dietary supplement and is destroyed by heat or reduced by prolonged storage (Weinstein et al., 2001). Humans, unlike most animals, are unable to synthesize vitamin C endogenously, so it is an essential dietary component (Naidu, 2003; Li, 2007). Sex steroids are hormones produced mainly by the reproductive glands, either the ovaries or testes, which share a similar basic structure of three hexane rings and a pentane ring (Gasbarri, 2010). The primary role of the gonadal steroid hormones in mammals is to regulate reproduction and related behaviours; however, both androgens and estrogens are also integrally involved in mediating higher brain function and processes, including cognition, neural development and neural plasticity (Dohanich, 2002). The hippocampal system plays an important role in memory function. Neurohormones like androgens and oestrogens are present in the hippocampus and have important roles in learning and memory (Talebi et al., 2010).

Oestrogens act on the central nervous system (CNS) both through genomic mechanisms, modulating synthesis, release and metabolism of neurotransmitters, neuropeptides and neurosteroids, and through non-genomic mechanisms, influencing electrical excitability, synaptic function and morphological features. Therefore, oestrogen’s neuroactive effects are multifaceted and encompass a system that ranges from the chemical to the biochemical to the genomic mechanisms, protecting against a wide range of neurotoxic insults (Genazzani et al., 2007).....

For more Human Physiology Project click here

This is a General Thesis for both Undergraduate & Postgraduate Studies. The complete research material plus questionnaire and references can be obtained at an affordable price of N3,000 within Nigerian or its equivalent in other currencies.


Kindly pay/transfer a total sum of N3,000 into any of our Bank Accounts listed below:
·         Diamond Bank Account:
A/C Name:      Haastrup Francis
A/C No.:         0096144450

·         GTBank Account:
A/C Name:      Haastrup Francis
A/C No.:         0029938679
After payment, send your desired Project Topic, Depositor’s Name, and your Active E-Mail Address to which the material would be sent for downloading (you can request for a downloading link if you don’t have an active email address) to +2348074521866 or +2348066484965. You can as well give us a direct phone call if you wish to. Projects materials are sent in Microsoft format to your mail within 30 Minutes once payment is confirmed.

N/B:    By ordering for our material means you have read and accepted our Terms and Conditions

Terms of Use: This is an academic paper. Students should NOT copy our materials word to word, as we DO NOT encourage Plagiarism. Only use as guide in developing your original research work.

Delivery Assurance
We are trustworthy and can never SCAM you. Our success story is based on the love and fear for God plus constant referrals from our clients who have benefited from our site. We deliver project materials to your Email address within 15-30 Minutes depending on how fast your payment is acknowledged by us.

Quality Assurance
All research projects, Research Term Papers and Essays on this site are well researched, supervised and approved by lecturers who are intellectuals in their various fields of study.

Search for your topic here

See full list of Project Topics under your Department Here!

Featured Post

Article: How to Write a Research Proposal

Most students and beginning researchers do not fully understand what a research proposal means, nor do they understand ...

Popular Posts