ANTICONVULSANT STUDIES OF THE METHANOL EXTRACT AND FRACTIONS OF CASSIA SIAMEA LAM. (FABACEAE) IN MICE AND CHICKS


For more Pharmacology & Therapeutics Project click here



TABLE OF CONTENT

Title page
Abstract
Table of Contents

CHAPTER ONE
1.0       INTRODUCTION
1.1       Statement of research problem
1.2       Justification
1.3       Theoretical framework
1.4       Aims and objectives
1.5       Research Hypothesis

CHAPTER TWO
2.0       LITERATURE REVIEW
2.1       Etiology and risk factors for developing Epilepsy
2.2       Classification of seizures
2.2.1    Partial seizures
2.2.2    Generalized seizures
2.2.3    Unclassified epileptic seizures
2.3       Mechanism of Epileptogenesis
2.3.1    Basic Mechanisms of Focal Seizure Initiation and Propagation
2.3.2    Gamma aminobutyric acid (GABA) - receptor Inhibition
2.3.3    Glutamate Receptors Activation
2.3.4    Extra-neuronal (extrinsic) factors
2.4       Diagnosis of Epilepsy
2.4.1    Electroencephalography
2.4.2    Neuro-imaging Computerized Tomography
2.4.3    Plain Radiology
2.4.4    Single Photon Emission Computerized Tomography (SPECT)
2.4.5.   Magnetic Resonance Imaging (MRI)
2.5       Classification of Antiepileptic drugs on the basis of their mechanism(s) of action
2.5.1    Voltage-Gated Sodium Channels blockers
2.5.2    Voltage-gated Calcium channels blockers
2.5.3    GABA ergic agents
2.5.4    Glutamate blockers
2.5.5    Carbonic anhydrase inhibitors
2.6       Limitations associated with the use of existing antiepileptic drugs
2.7.1    Medicinal plants and Epilepsy
2.7       Plant description: Cassia siamea
2.6.1    Botanical description
2.6.2    Taxonomic classification
2.6.3    Ethnomedical uses of Cassia siamea plant in

CHAPTER THREE
3.0       MATERIALS AND METHODS
3.1       MATERIALS
3.1.1    Plant Material collection and Authentication
3.2       Experimental animals
3.3       Drug and chemicals
3.4       Equipment
3.5       Fractionation of Cassia siamea crude extract
3.6       Preliminary phytochemical screening
3.3       Pharmacological Studies
3.3.1    Acute toxicity Studies (LD50)
3.3.2    Anticonvulsant studies
3.3.2.1 Maximal electroshock-induced seizure test in chicks
3.3.2.2 Pentylenetetrazole –induced convulsion in mice
3.3.2.3 Picrotoxin-induced seizure in mice
3.3.2.4 Effect of naloxone on the anticonvulsant activity of the Ethyl acetate fraction
3.4       Behavioral study on the Ethylacetate fraction of Cassia siamea plant extract
3.4.1    Diazepam-induced Sleep test in Mice
3.6       Statistical Analysis

CHAPTER FOUR
4.0       RESULTS
4.1.      The Percentage yield of the Plant Extract and Fractions
4.1.1    Fractionation of the Crude Methanol Whole Plant Extract
4.2       Phytochemical constituents of the Whole Plant Extract of Cassia siamea
4.3       LD50 Value Determination in Mice and Day-Old Chicks
4.4       Anticonvulsant Studies
4.4.1    Maximal Electroshock-induced Seizures in Chicks
4.4.2    Pentylenetetrazole- induced seizures in mice
4.4.3    Effect of the Ethyl acetate fraction of the Methanol Extract of Cassia siamea on Picrotoxin-induced Seizure in Mice
4.4.4    Effect of Naloxone on anticonvulsant activity of the Ethyl acetate portion of Cassia siamea on Pentylenetetrazole (S.cPTZ) –induced convulsion in mice
4.4.5    Effect of the Ethyl acetate fraction of Cassia siamea on Diazepam induced Sleep

CHAPTER FIVE
5.0       DISCUSSION


CHAPTER SIX
6.0       SUMMARY, CONCLUSION AND RECOMMENDATIONS
REFERENCES




ABSTRACT

Cassia siamea is a shrub belonging to the Fabaceae family, native of Southeast Asia. The plant is commonly used in traditional medicine to treat hypertension, malaria and diabetes. Due to the easy cultivation of the plant, its widespread and also remarkable biological activities, Cassia siamea has become a worldwide medicine. The study was conducted to assess the anticonvulsant potential of the methanol extract of Cassia siamea (CSME) and its fractions (EAF, NBF and RAF) using Pentylenetetrazole (PTZ) induced seizures in mice and Maximal electroshock (MES) induced seizures in chicks. Acute toxicity was carried out on CSME and fractions. The possible mechanism(s) involved in anticonvulsant action were determined using Picrotoxin and naloxone. The preliminary phytochemical screening of the methanol extract revealed the presence of alkaloids, flavonoids, polyphenols, saponins, steroids, terpenoids and tannins. The LD50 of CSME, its EAF and NBF was found to be greater than 5000mg/kg intraperitoneally (i.p) in chicks and mice, suggesting a non toxic profile of the extractst, while the LD50 of the RAF (i.p) was found to be 1095mg/kg in mice and 3807 mg/kg in chicks implying the RAF is mildly toxic via the intraperitoneal route. The CSME and its EAF were found to have varied anticonvulsant activities; the EAF at 250mg/kg dose protected 40% of mice against hind limb tonic extension induced by maximal electroshock and in convulsed chicks a significant (P < 0.05) decrease in mean recovery time was noted. The ethyl acetate fraction at 250mg/kg and 500mg/kg doses produced a significant (P < 0.05) delay in mean onset of seizures and offered mice a 2/6 and 5/6 quantal protection against mortality, valproic acid the standard anticonvulsant drug used produced a 100% protection against seizures. The EAF did not protect mice against pirotoxin induced seizures indicating lack of activity on chloride ion channels of the GABAA receptor complex. Naloxone did not reverse the anticonvulsant activity of the EAF against Pentylenetetrazole-induced seizures, suggesting lack ogf involvement of GABAA-BDZ receptors and opioid receptors in the anticonvulsant effect of EAF. The CSME and EAF at 100mg/kg dose significantly (P < 0.05) prolonged the total duration of Diazepam induced sleeping time in mice without affecting the mean onset of sleep, indicating sedative action of the extract. The results suggests the presence of bioactive component(s) that posess anticonvulsant and sedative activities. The data may provide pharmacological basis for the use of the plant alone or in combination with other plant(s) in the management of febrile convulsions and insomnia in West African countries including Nigeria.





CHAPTER ONE


1.0  INTRODUCTION

1.1  Background information




Epilepsy is one of the most common afflictions of human beings (Muralidharan et al., 2009). It affects approximately 50 million people worldwide and accounts for about 1% of the global burden of disease (Reynolds, 2002). Epilepsy is coined from the Greek word

epilembanein” which means "to seize”. It is a chronic neurological disorder affecting both sexes (Blume et al., 2001). It is the second most common chronic neurological condition seen by neurologists worldwide (Sridharan, 2002). It is characterized predominantly by recurrent and unpredictable interruptions of normal brain function, called epileptic seizures which arise due to sudden, excessive and rapid discharge of cerebral neurons in the grey

matter of the brain. It is a diverse family of disorders, having in common an abnormally increased predisposition to seizures reflecting underlying brain dysfunction that may result from different causes (Fischer et al., 2005).


Among brain disorders, epilepsy stands out not only because of its high prevalence and incidence rates, but in particular because of the myths and beliefs attached to the condition in various cultures and the resulting impacts on the individual, the family, and the community as a whole (Jamison et al., 2006). Epilepsy remains among the most stigmatized brain disorders. The associated stigma is more obvious in developing countries because of illiteracy and misinformation regarding the actual nature of the condition......

For more Pharmacology & Therapeutics Project click here


___________________________________________________________________________
This is a General Thesis for both Undergraduate & Postgraduate Studies. The complete research material plus questionnaire and references can be obtained at an affordable price of N3,000 within Nigerian or its equivalent in other currencies.


INSTRUCTION ON HOW TO GET THE COMPLETE PROJECT MATERIAL

Kindly pay/transfer a total sum of N3,000 into any of our Bank Accounts listed below:
·         Diamond Bank Account:
A/C Name:      Haastrup Francis
A/C No.:         0096144450

·         GTBank Account:
A/C Name:      Haastrup Francis
A/C No.:         0029938679

After payment, send your desired Project Topic, Depositor’s Name, and your Active E-Mail Address to which the material would be sent for downloading (you can request for a downloading link if you don’t have an active email address) to +2348074521866 or +2348066484965. You can as well give us a direct phone call if you wish to. Projects materials are sent in Microsoft format to your mail within 30 Minutes once payment is confirmed. 

--------------------------------------------------------
N/B:    By ordering for our material means you have read and accepted our Terms and Conditions


Terms of Use: This is an academic paper. Students should NOT copy our materials word to word, as we DO NOT encourage Plagiarism. Only use as guide in developing your original research work.

Delivery Assurance
We are trustworthy and can never SCAM you. Our success story is based on the love and fear for God plus constant referrals from our clients who have benefited from our site. We deliver project materials to your Email address within 15-30 Minutes depending on how fast your payment is acknowledged by us.

Quality Assurance
All research projects, Research Term Papers and Essays on this site are well researched, supervised and approved by lecturers who are intellectuals in their various fields of study.
Share:

Search for your topic here

To view a full list of Project Topics under your Department

Featured Post

Article: How to Write a Research Proposal

Most students and beginning researchers do not fully understand what a research proposal means, nor do they understand ...

Popular Posts